Java Tea（Orthosiphon stamineus）Extract

• Product Detail

Product name	Java Tea（Orthosiphon stamineus）Extract
Latin Name	Orthosiphon stamineus
Active ingredients	Sinensetin
synonyms	Arjak, Barbiflore, Clerodendranthus spicatus, Clerodendrum spicatum, Java Thé, Moustaches de Chat, Ocimum aristatum, Orthosiphon, Orthosiphon aristatus, Orthosiphon Grandiflorus, Orthosiphon spicatus, Orthosiphon stamineus, Orthosiphonis Folium, Té de Java, Thé de Java, Vantulsi，O. stamineus
Appearance	Light yellow brown fine powder
Part used	Herb
Specification	5:1, 10:1, Sinensetin 1.2%, Sinensetin 0.5%
Dosage	110mg-8100mg daily
Main benefits	Antihyperglycemic, Antihypertensive, Renal lithiasis
Applied industries	Medicine, food additive, dietary supplement,

What is Java Tea（Orthosiphon stamineus）Extract?

Orthosiphon stamineus is a traditional herb that is widely grown in tropical areas. It is also known as Orthosiphon aristatus. The plant can be distinguished by its white or purple colored flowers that resembles cat whiskers. The herb is popularly known as Java tea. It is also commonly referred as "Misai Kucing" which means cat whiskers. O. stamineus is used widely in the form of herbal tea among the South East Asian population . Java tea was possibly introduced to the West in the early 20th century. The brewing of Java tea is similar to that for other teas. It is soaked in hot boiling water for about three minutes, and honey or milk is then added. It can be easily prepared as garden tea from the dried leaves. There are quite a number of commercial products derived from Misai Kucing. Cultivation areas and post-harvesting method can significantly affect the quality of the herb.

Chemical constituents of Java Tea（Orthosiphon stamineus）Extract

The phytochemical study of kumis kucing grown in Asia have been

conducted extensively since the 1930’s. More than hundreds

chemical compounds were reported and classified as monoterpenes,

diterpenes, triterpenes, saponins, flavonoids, organic acids, and etc

e essential oil of leaves of O. Stamineus. They were β-Carryophyll α-Humulene,  β-Elemene,  1-Octen-3-ol,  β-Bourbene,  β-Pinene,Phenylacetaldehyde, Caryophyllene oxide, Camphene, cis-2-Octenal, 3-Octanol,  Limonene,  2-Pentenyl  furane,  Hexanal,  Naphtalene,

Benzaldehyde, trans 2-Hexanal, Heptenal, trans cis-Octa-3-5-dien-2-one, Decanal, δ-Elemene, 1,8-Cineol, 4-Heptenal, Isomenthone,chavicol,  α-Pinene,Tridecan,  ρ-Cymene,  Camphor,  1-

Methylnaphtalene, α-Muniolene, trans trans-Octa-3-5-dien-2-one, 2-Amylfurane, Menthone, Carvone, Cittonellol, α-Copaene, Borneol,Dodecane, Eugenol, Linalool, trans-Linalooloxide, δ-Cadipene, trans-2-(cis)-6- Nonadienale, Methyleugenol, α-Gubebene, Geranylacetane, δ-Terpineol, Acetophenone, trans- Anethol, Germacrene D, β-Cyclocitral,Damascenone, Dehydroionone, cis-Linalooloxide, Undecan, BornylAcetate,  2-Methylnapthalene,  β-Ionone,  Perillen,  Safranal,Hexahydrofamesylacetone,Hexan-1-ol,2,6,6-Trimethyl-2-cyclohexe-

1,4-dione,  Isobornylacetate,  trans-Deca-2,4-dienal,  cis-Caryophylene, Germacrene, and cis-3-Hexen-1-ol .

Now use most of the Sinensetin.

Benefits of taking Java Tea（Orthosiphon stamineus）Extract supplements:

Traditional medicine

Orthosiphon stamineus (OS) popularly known as "diuretic agent" are traditionally used in folk medicine in the treatment of hyperuricemia, rheumatism, gout, nephritis, nephrolithiasis, urethritis and cystitis.

Modern application

1. Java Tea（Orthosiphon stamineus）Extract and Antihyperglycemic

>Antihyperglycemic activity of functional drinks based on Java Tea

(Orthosiphon aristatus) in streptozotocin induced diabetic mice

Biopharmaca Research Center, Bogor Agricultural University, Bogor 16128, Indonesia;

Abstract

Diabetes is a group of diseases marked by high levels of blood glucose resulting from defects

in insulin production, insulin action, or both. Diabetes can lead to serious complications and

premature death. Antioxidant compounds in functional drinks such as flavonoid may offer some

protection against the early stage of diabetic mellitus and the development of complications.

The objective of this study was to investigate the antihyperglycemic effects of functional drinks

formulations containing different varieties of Java Tea on streptozotocin induced diabetic mice.

The results indicated that the administration of functional drinks in diabetic mice restrain the

increase of blood glucose and further inhibit the rate of pancreatic beta cells damage. HPLC

analysis shows that the bioactive compounds in the extract ingredient are sinensetin, 6-gingerol,

8-gingerol, 10-gingerol, 6-shogaol, curcumin, desmethoxycurcumin, brazilin, hesperidin and

naringin. Consequently, functional drinks based on Java Tea is considered to be a promising

functional drink for preventing and treating diabetes

2. Java Tea（Orthosiphon stamineus）Extract and Antihypertensive

> Antihypertensive actions of methylripariochromene A from Orthosiphon aristatus, an Indonesian traditional medicinal plant.

Matsubara T1, Bohgaki T, Watarai M, Suzuki H, Ohashi K, Shibuya H.

Abstract

Methylripariochromene A (MRC) was isolated from the leaves of Orthosiphon aristatus (Lamiaceae) and subjected to the examination of several pharmacological actions related to antihypertensive activity. The following four findings were revealed from the present study: 1) MRC caused a continuous decrease in systolic blood pressure and a decrease in heart rate after subcutaneous administration in conscious male SHRSP, 2) MRC exhibited the concentration-dependent suppression of contractions induced by high K+, l-phenylephrine or prostaglandin F2alpha in endothelium-denuded rat thoracic aorta, 3) MRC showed a marked suppression of contractile force without a significant reduction in the beating rate in isolated bilateral guinea pig atria, and 4) MRC increased urinary volume and the excretion of Na+, K+ and Cl- for 3 h after oral administration with a load of saline in fasted rats. These findings indicate that MRC possesses some actions related to a decrease in blood pressure, i.e. vasodilating action, a decrease in cardiac output and diuretic action. Furthermore, it is presumed that the traditional use of this plant in the therapy of hypertension may be partially supported by these actions with MRC.

> Antihypertensive actions of methylripariochromene A from Orthosiphon aristatus, an Indonesian traditional medicinal plant.

Matsubara T1, Bohgaki T, Watarai M, Suzuki H, Ohashi K, Shibuya H.

Abstract

Methylripariochromene A (MRC) was isolated from the leaves of Orthosiphon aristatus (Lamiaceae) and subjected to the examination of several pharmacological actions related to antihypertensive activity. The following four findings were revealed from the present study: 1) MRC caused a continuous decrease in systolic blood pressure and a decrease in heart rate after subcutaneous administration in conscious male SHRSP, 2) MRC exhibited the concentration-dependent suppression of contractions induced by high K+, l-phenylephrine or prostaglandin F2alpha in endothelium-denuded rat thoracic aorta, 3) MRC showed a marked suppression of contractile force without a significant reduction in the beating rate in isolated bilateral guinea pig atria, and 4) MRC increased urinary volume and the excretion of Na+, K+ and Cl- for 3 h after oral administration with a load of saline in fasted rats. These findings indicate that MRC possesses some actions related to a decrease in blood pressure, i.e. vasodilating action, a decrease in cardiac output and diuretic action. Furthermore, it is presumed that the traditional use of this plant in the therapy of hypertension may be partially supported by these actions with MRC.

3. Java Tea（Orthosiphon stamineus）Extract and Prostate Cancer Cells

Optimization of Cat's Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells.

Al-Suede FS1, Khadeer Ahamed MB1, Abdul Majid AS2, Baharetha HM1, Hassan LE1, Kadir MO3, Nassar ZD4, Abdul Majid AM1.

Abstract

Cat's whiskers (Orthosiphon stamineus) leaves extracts were prepared using supercritical CO2 (SC-CO2) with full factorial design to determine the optimum extraction parameters. Nine extracts were obtained by varying pressure, temperature, and time. The extracts were analysed using FTIR, UV-Vis, and GC-MS. Cytotoxicity of the extracts was evaluated on human (colorectal, breast, and prostate) cancer and normal fibroblast cells. Moderate pressure (31.1 MPa) and temperature (60°C) were recorded as optimum extraction conditions with high yield (1.74%) of the extract (B2) at 60 min extraction time. The optimized extract (B2) displayed selective cytotoxicity against prostate cancer (PC3) cells (IC50 28 µg/mL) and significant antioxidant activity (IC50 42.8 µg/mL). Elevated levels of caspases 3/7 and 9 in B2-treated PC3 cells suggest the induction of apoptosis through nuclear and mitochondrial pathways. Hoechst and rhodamine assays confirmed the nuclear condensation and disruption of mitochondrial membrane potential in the cells. B2 also demonstrated inhibitory effects on motility and colonies of PC3 cells at its subcytotoxic concentrations. It is noteworthy that B2 displayed negligible toxicity against the normal cells. Chemometric analysis revealed high content of essential oils, hydrocarbon, fatty acids, esters, and aromatic sesquiterpenes in B2. This study highlights the therapeutic potentials of SC-CO2 extract of cat's whiskers in targeting prostate carcinoma.

4. Java Tea（Orthosiphon stamineus）Extract and Renal lithiasis

>Adenosine A1 receptor binding activity of methoxy flavonoids from Orthosiphon stamineus.

Yuliana ND1, Khatib A, Link-Struensee AM, Ijzerman AP, Rungkat-Zakaria F, Choi YH, Verpoorte R.

Abstract

Orthosiphon stamineus Benth. ( Orthosiphon Grandiflorus Bold. or Clerodendranthus spicatus Thunb.) is an Indonesian medicinal herb traditionally used for diseases such as hypertension, diabetes, and kidney stones. Despite the importance of this last application, there are very few reports on it. Diuretic action is an important factor in kidney stone treatment, as an increase in the volume of fluid flowing through the kidney will help to dissolve the stones, assist their passing to avoid further retention, and flush out the deposits. Among the diverse roles of adenosine A (1) receptor antagonists in renal protection, many studies have shown that they can induce diuresis and sodium excretion. A bioassay-guided fractionation of a methanol-water extract of Orthosiphon stamineus leaves using the adenosine A (1) receptor binding assay resulted in the isolation of seven methoxy flavonoids as active ligands with K(i) values in the micromolar range. The Hill slope values are not significantly different from unity (within 0.9 - 1.4), which indicates the antagonist effect to A (1)-R. The results of this study thus provide a scientific foundation for the traditional use ofOrthosiphon stamineus in kidney stone treatment, as the affinity of the active compounds isolated from it as adenosine A (1) receptor ligands allows them to be associated with diuretic activity, which is one possible treatment for renal lithiasis.

Side effects and safety of Java Tea（Orthosiphon stamineus）Extract

 >Antioxidant and toxicity studies of 50% methanolic extract of Orthosiphon stamineus Benth.

Yam MF1, Lim CP1, Fung Ang L1, Por LY2, Wong ST3, Asmawi MZ1, Basir R4, Ahmad M1.

Author information

Abstract

The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus (Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract. In acute toxicity study, single dose of MEOS, 5000 mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14 days. While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000 mg/kg/day for 28 days. From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating, and antilipid peroxidation activities. There was no mortality detected or any signs of toxicity in acute and subchronic toxicity studies. Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical parameters between both male and female treated rats in any doses tested. No abnormality of internal organs was observed between treatment and control groups. The oral lethal dose determined was more than 5000 mg/kg and the no-observed-adverse-effect level (NOAEL) of MEOS for both male and female rats is considered to be 5000 mg/kg per day.

Dosage of Java Tea（Orthosiphon stamineus）Extract

supplement:

There is not enough information to get an ideal dose, but at this moment it seems that a 70% ethanolic extract would be the most prudent supplemental dosage for the production of leptin in the body. Since this has been noted in a highly variable range (as low as 20mg/kg in mice, as high as 450mg/kg in rats) it is uncertain what the best oral dose to take would be. Nonetheless, human equivalents for the above range are:

l  110-4,900 mg for a 150lb person

l  150-6,500 mg for a 200lb person

l  180-8,100 mg for a 250lb person

The diuretic effect has been noted with tea, and thus brewing a tea from the leaves may be sufficient.

Consult your physician if you have any questions about the proper use of Java Tea（Orthosiphon stamineus）Extract supplements.

Company Information:

Nutragreen Biotechnology Co., Ltd, a brand of Shanghai Lvshang Biotech Co., Ltd, is a GMP compliant and FDA registered manufacturer and supplier of raw materials of plant extracts, botanicals, herbs, especially Tradtional Chinese herbs. Java Tea（Orthosiphon stamineus）Extract is one of our most competitive ingredients with various specifications and stocks available all year round. You may leave a message below for more detailed information.